goat polyclonal antibodies  (R&D Systems)

Journal: eLife

Article Title: Dynamics of compartment-specific proteomic landscapes of hepatotoxic and cholestatic models of liver fibrosis

doi: 10.7554/eLife.98023

Figure Lengend Snippet:

Article Snippet: The following primary antibodies were used: rat mAb to K19 (Troma III; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA, USA); mouse mAbs to αSMA (DAKO, Glostrup, Denmark) and ECM1 (Santa Cruz Biotechnology, Dallas, TX, USA), goat polyclonal antibodies to clusterin (R&D Systems, Minneapolis, MN, USA), rabbit polyclonal antibodies to fibronectin, collagen type I, collagen type IV, and monoclonal to integrin αV (all from Abcam, Cambridge, UK).

Techniques: Software, Staining

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation Pattern of Serum Clusterin in Psoriatic Arthritis and Rheumatoid Arthritis—The Search for New Diagnostic Glycomarkers

doi: 10.3390/ijms252313060

Figure Lengend Snippet: The parameter values of blood serum CLU glycosylation analysis.

Article Snippet: ELISA plates (Nunc MaxiSorp, Thermo Fisher Scientific, Roskilde, Denmark) were coated with goat anti-human clusterin polyclonal antibodies (Invitrogen, Thermo Fisher Scientific, catalog No. PA1-26903; Rockford, IL, USA).

Techniques: Glycoproteomics, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation Pattern of Serum Clusterin in Psoriatic Arthritis and Rheumatoid Arthritis—The Search for New Diagnostic Glycomarkers

doi: 10.3390/ijms252313060

Figure Lengend Snippet: The correlations between examined parameters. Correlated parameters: CLU vs. LTA ( A ), CLU vs. UEA ( B ), MAA vs. LTA ( C ), MAA vs. UEA ( D ), MAA vs. LCA ( E ), UEA vs. SNA ( F ), UEA vs. LTA ( G ), LCA vs. LTA ( H ), and LCA vs. UEA ( I ). CLU—clusterin concentration in µg/mL; SNA—relative reactivity of blood serum CLU glycans with Sambucus nigra agglutinin; MAA—relative reactivity of blood serum CLU glycans with Maackia amurensis agglutinin; LTA—relative reactivity of blood serum CLU glycans with Lotus tetragonolobus agglutinin; UEA—relative reactivity of blood serum CLU glycans with Ulex europaeus agglutinin; LCA—relative reactivity of blood serum CLU glycans with Lens culinaris agglutinin. Specificities of lectins are presented in . Relative reactivities of CLU glycans with lectins are expressed in absorbance units (AU). The correlations were calculated using a Spearman’s rank test, and a two-tailed p -value (probability value) of less than 0.05 was considered significant. Blue diamonds—a set of points, each of which has the value of one variable defining the position on the horizontal axis and the value of a second variable defining the position on the vertical axis. Line of best fit (trend line) is marked in red. The red dashed line points to 95% of the confidence interval.

Article Snippet: ELISA plates (Nunc MaxiSorp, Thermo Fisher Scientific, Roskilde, Denmark) were coated with goat anti-human clusterin polyclonal antibodies (Invitrogen, Thermo Fisher Scientific, catalog No. PA1-26903; Rockford, IL, USA).

Techniques: Concentration Assay, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation Pattern of Serum Clusterin in Psoriatic Arthritis and Rheumatoid Arthritis—The Search for New Diagnostic Glycomarkers

doi: 10.3390/ijms252313060

Figure Lengend Snippet: The results of receiver operating characteristic (ROC) curves analysis for relative reactivities of blood serum clusterin with lectins.

Article Snippet: ELISA plates (Nunc MaxiSorp, Thermo Fisher Scientific, Roskilde, Denmark) were coated with goat anti-human clusterin polyclonal antibodies (Invitrogen, Thermo Fisher Scientific, catalog No. PA1-26903; Rockford, IL, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation Pattern of Serum Clusterin in Psoriatic Arthritis and Rheumatoid Arthritis—The Search for New Diagnostic Glycomarkers

doi: 10.3390/ijms252313060

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for CLU concentrations and relative reactivities of blood serum CLU glycans with lectins for which the area under the curve (AUC) was higher than 0.700. C—control; PsA—psoriatic arthritis; RA—rheumatoid arthritis; CLU—clusterin concentration in µg/mL; SNA—relative reactivity of blood serum CLU glycans with Sambucus nigra agglutinin. SNA specificity is presented in . Relative reactivities of CLU glycans with lectin are expressed in absorbance units (AU). The reference line is marked in red, the receiver operating characteristics for an analyzed parameter in blue, and the cut-off point in green.

Article Snippet: ELISA plates (Nunc MaxiSorp, Thermo Fisher Scientific, Roskilde, Denmark) were coated with goat anti-human clusterin polyclonal antibodies (Invitrogen, Thermo Fisher Scientific, catalog No. PA1-26903; Rockford, IL, USA).

Techniques: Control, Concentration Assay